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Determination of codeine and its metabolites in human blood plasma and in microsomal incubates
Summary
Type:
HPLC
Application #:
101210
Catalog#:
720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection:
UV 214nm
Conditions:
eluent: 7% (v/v) acetonitrile - 10mM phosphate buffer pH 2.1 - 2mM propyl sulphate
Authors:
Persson K
Source:
J Chromatogr 491 (1989) 473 - 480
Details
Legend: Chromatogram of (left) blank and (right) plasma sample spiked with 25ng/ml morphine, 25ng/ml norcodeine (NC), and 50ng/ml codeine (COD), sample extracted with dichloromethane containing 15% 2-propan
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